Protein Purification Reagents and Kits
STEMCELL Technologies RosetteSep™ Human Cord Blood Debulking Cocktail, For processing: 200 mL cord blood processed
The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Mouse APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
Active Motif Histone Extraction Kit
The Histone Extraction Kit provides a simple method to acid precipitate histones from cell culture, tissue and primary cells (e.g. T-cells) while preserving histone post-translational modifications. The highly basic nature of histone proteins often precludes them from being isolated using traditional methods for protein extraction. Instead, histones must be isolated under acidic conditions to retain solubility. The inclusion of inhibitors helps to preserve histone modifications such as acetylation, methylation and phosphorylation. Each Histone Extraction Kit contains enough reagents for extractions from 2 x 96-well plates (192 rxns), or 100 rxns from cultured cell lines and tissues. Kits include buffers, protease inhibitors, phosphatase inhibitors, deacetylase inhibitors and optimized protocols for efficient recovery of core histones (H2A, H2B, H3 & H4). For more complete information, please visit the Active Motif website.
Zymo Research Corporation Zymo-Spin™ ChIP Kit (25 preps) Mechanical Shearing Protocol '*'
This ChIP protocol involves covalent cross-linking of protein-DNA complexes with formaldehyde followed by cell lysis and chromatin shearing. A ChIP-grade antibody (user supplied) is used with Protein A magnetic beads to immunoprecipitate the protein-DNA complexes of interest. Following reverse crosslinking, RNase A and Proteinase K treatments, the DNA is eluted in a minimal volume of buffer using a unique micro-elution spin column, eliminating the need for messy precipitations. The protocol has been optimized for efficient crosslinking, shearing, and immunoprecipitation regardless of the mammalian cell type. Additionally, eluted ChIP DNA is ideal for end point PCR, quantitative PCR, ChIPSeq library preparation, and Next-Gen sequencing-based applications.
Medchemexpress LLC MUSK Mouse Myc Hi 5ug
Recombinant mouse MUSK protein bearing N-terminal Myc and His-SUMO tags, supplied lyophilized for biochemical and biophysical studies. The product is a fragment corresponding to residues E22-S494, expressed in E. coli, and formulated for stability and downstream assays such as SPR after buffer exchange.
- Recombinant fragment covering residues E22-S494.
- Expressed in E. coli and supplied lyophilized for long-term storage.
- Endotoxin level below 1 EU/μg as measured by LAL.
- Lyophilized from Tris-EDTA with trehalose for enhanced stability.
- Recommended reconstitution at not less than 100 μg/mL; buffer exchange suggested for SPR.
- Approximate molecular weight 69.3 kDa.
Echelon Biosciences Research Labs PI(3,4,5)P2 PIP Beads 1 mL
PI(3,4,5)P3 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,4,5)P3 per mL of beads, enough for several protein binding experiment. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. This item is non returnable
Takara Bio HISTALONA GRAVITY COLUMN KIT
635654 HisTALON(TM) Gravity Column Purification Kit 5 x 1 mL Columns
Hampton Research STOCKOPT MALIC ACID #23 PH 5.9
HR2-240-23/StockOptions Malic Acid #23, 1.0 M DL-Malic acid pH 5.9, 185 ml. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #71 (F11)
HR2-072-71/Solubility & Stability #71 (F11), 1.0 ml - 250 mM Sodium p-toluenesulfonate. Please note items from this supplier are non-returnable.
Hampton Research SOL & STABILITY #69 (F9)
HR2-072-69/Solubility & Stability #69 (F09), 1.0 ml - 250 mM Ammonium acetate. Please note items from this supplier are non-returnable.